Product Description

Estrogen Receptor α Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20757-50, ES20757-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined. [provided by RefSeq, Mar 2014],

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB

Alternate Names: Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)

Clonality: Polyclonal

Immunogen: Recombinant Protein of Estrogen Receptor α

Isotype: IgG

Target: Estrogen Receptor-α

Specificity: The antibody detects endogenous Estrogen Receptor α protein

Dilution: WB: 1:500-1:2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed (PubMed:10970861), Not expressed in the pituitary gland (PubMed:10970861),, [Isoform 3]: Widely expressed, however not expressed in the pituitary gland,

Observed Band (KD): 66kD

Fields: Endocrine resistance, Estrogen signaling pathway, Prolactin signaling pathway, Thyroid hormone signaling pathway, Endocrine and other factor-regulated calcium reabsorption, Pathways in cancer, Proteoglycans in cancer, Chemical carcinogenesis - receptor activation, Breast cancer

Function: Domain: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain
Function: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues
Online Information: Estrogen receptor entry,polymorphism: Genetic variations in ESR1 are correlated with bone mineral density (BMD). Low BMD is a risk factor for osteoporotic fracture. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture
PTM: Glycosylated, contains N-acetylglucosamine, probably O-linked
PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcri

Subcellular Location: [Isoform 1]: Nucleus Cytoplasm Cell membrane, Peripheral membrane protein, Cytoplasmic side A minor fraction is associated with the inner membrane, [Isoform 3]: Nucleus Cytoplasm Cell membrane, Peripheral membrane protein, Cytoplasmic side Cell membrane, Single-pass type I membrane protein Associated with the inner membrane via palmitoylation (Probable) At least a subset exists as a transmembrane protein with a N-terminal extracellular domain, Nucleus Golgi apparatus Cell membrane Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs Associated with the plasma membrane when palmitoylated

Storage and Stability: -20°C/1 year

Gene Name: ESR1

Protein Name: Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)

Mouse SwissProt Number: P19785

Rat SwissProt Number: P06211

Research Use Only