Product Description

Estrogen Receptor alpha Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66133-50, MB66133-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Estrogen receptor α (ERα), a member of the steroid receptor superfamily, contains highly conserved DNA binding and ligand binding domains . Through its estrogen-independent and estrogen-dependent activation domains (AF-1 and AF-2, respectively), ERα regμlates transcription by recruiting coactivator proteins and interacting with general transcriptional machinery. Phosphorylation at mμltiple sites provides an important mechanism to regμlate ERα activity . Ser104, 106, 118, and 167 are located in the amino-terminal transcription activation function domain AF-1, and phosphorylation of these serine residues plays an important role in regμlating ERα activity. Ser118 may be the substrate of the transcription regμlatory kinase CDK7 . Ser167 may be phosphorylated by p90RSK and Akt . According to the research literature, phosphorylation at Ser167 may confer tamoxifen resistance in breast cancer patients .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: IHC

Alternate Names: ESR, NR3A1, Estrogen receptor, ER, ER-alpha, Estradiol receptor, Nuclear receptor subfamily 3 group A member 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Estrogen Receptor alpha. The exact sequence is proprietary.

Dilution: IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Estrogen Receptor alpha protein.

Molecular Weight: ~ 66 kDa

Pathway: MAPK Erk in Growth and Differentiation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P03372

Product: Mouse IgG2b. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only