Ethanol Colorimetric Assay Kit
Sizes: 48T, 96T, 500 Assays
Catalogue Numbers: E-BC-K891-M-48, E-BC-K891-M-96, E-BC-K891-M-500
Citations, Manuals and MSDS Available upon request.
Research Areas: Liver and Renal Function
Result Type: Quantitative
Detection Instrument: Microplate reader
Tested Sample Types: serum, plasma, wine
Assay Time: 30 min
Detection Type: Colorimetric method
Sensitivity: 0.27 μmol/mL
Detection Range: 0.27–10.0 μmol/mL
Test Principle: Ethanol dehydrogenase can catalyze oxidative dehydrogenation of ethanol to acetaldehyde, and NAD+ is reduced to produce NADH. NADH, under the action of 1-mPMS, transfer electrons to WST-8 to produce the yellow product, which has a characteristic absorption peak at 450 nm. Therefore, ethanol content can be quantified by measure the OD value at 450 nm.
Inter CV: 5
Intra CV: 3.5
Storage: This product can be stored at -20°C for 12 months with shading light.
Shipping: Ice bag