Product Description

ETK (K35) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1536-50, BS1536-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P51813

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: ETK, also known as BMX, is a member of the Bruton's tyrosine kinase (Btk) family. It is expressed in a variety of hematopoietic, epithelial and endothelial cells. Etk, like other Btk family members, contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. It participates in multiple signal transduction pathways. Phosphorylation of Tyr566 by Src kinase is required for activation of Etk in vivo. In endothelial and epithelial cells, Etk is regulated by FAK through phosphorylation at Tyr40.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ETK (K35) polyclonal antibody detects endogenous levels of ETK protein.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of ETK (K35) pAb in paraffin-embeddedhuman liver carcinoma tissue at 1:5000. cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of ETK (K35) pAb in paraffin-embeddedhuman rectum carcinoma tissue at 1:5000. cytoplasmic and cell membrane staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Cytoplasmic tyrosine-protein kinase BMX; Bone marrow tyrosine kinase gene in chromosome X protein; Epithelial and endothelial tyrosine kinase; NTK38; BMX

Immunogen: Synthetic peptide, corresponding to amino acids N-terminus of Human ETK.

Conjugate: Unconjugated

Modification: Unmodified