Product Description

Ezh2 (phospho-Thr311) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES16646-50, ES16646-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: enhancer of zeste 2 polycomb repressive complex 2 subunit(EZH2) Homo sapiens This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Multiple alternatively splcied transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Feb 2011],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB

Alternate Names: Histone-lysine N-methyltransferase EZH2 (EC 2.1.1.43) (ENX-1) (Enhancer of zeste homolog 2) (Lysine N-methyltransferase 6)

Clonality: Polyclonal

Immunogen: Synthesized phosho peptide around human Ezh2 (Thr311)

Isotype: IgG

Target: Ezh2,

Specificity: This antibody detects endogenous levels of Human Ezh2 (phospho-Thr311)

Dilution: WB 1:1000-2000

Purification: The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: In the ovary, expressed in primordial follicles and oocytes and also in external follicle cells (at protein level) (PubMed:31451685). Expressed in many tissues (PubMed:14532106). Overexpressed in numerous tumor types including carcinomas of the breast, colon, larynx, lymphoma and testis (PubMed:14532106).

Observed Band (KD): 82kD

Fields: Lysine degradation, Metabolic pathways, MicroRNAs in cancer

Function: Catalytic Activity: S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine. Caution: Two variants of the PRC2 complex have been described, termed PRC3 and PRC4. Each of the three complexes may include a different complement of EED isoforms, although the precise sequences of the isoforms in each complex have not been determined. The PRC2 and PRC4 complexes may also methylate 'Lys-26' of histone H1 in addition to 'Lys-27' of histone H3 (PubMed:15099518 and PubMed:15684044), although other studies have demonstrated no methylation of 'Lys-26' of histone H1 by PRC2 (PubMed:16431907). Developmental Stage: Expression decreases during senescence of embryonic fibroblasts (HEFs). Expression peaks at the G1/S phase boundary. Polycomb group (PcG) protein. Catalytic subunit of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of hist

Subcellular Location: Nucleus Localizes to the inactive X chromosome in trophoblast stem cells.

Storage and Stability: -20°C/1 year

Gene Name: EZH2, KMT6

Protein Name: Ezh2, Thr311

Human Gene ID: 2146

Human SwissProt Number: Q15910

Mouse Gene ID: 14056

Mouse SwissProt Number: Q61188

Research Use Only