Product Description

FCF1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77119-50, BS77119-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9Y324

Host: Rabbit

Reactivity: Human, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: Essential protein involved in pre-rRNA processing and 40S ribosomal subunit assembly.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 23kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293T cells, using FCF1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 100s., Immunohistochemistry of paraffin-embedded Human esophageal using FCF1 Rabbit pAb at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of C6 cells using FCF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U-2 OS cells using FCF1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: FCF1; Bka; C14orf111; CGI-35; UTP24

Immunogen: A synthetic peptide of human FCF1(NP_057046.1).

Conjugate: Unconjugated

Modification: Unmodified