Product Description

FGF 22 (A96) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3026-50, BS3026-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9HCT0

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Acidic and basic fibroblast growth factors (FGFs) are members of a family of multifunctional polypeptide growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Like other growth factors, FGFs act by binding and activating specific cell surface receptors. These receptors usually contain an extracellular ligand-binding region containing three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic tyrosine kinase domain. Fibroblast growth factor- 22 (FGF-22) is a secreted protein whose gene is localized to human chromosome 19p13.3. FGF-22 is expressed preferentially in the inner root sheath of the hair follicle, suggesting that it is important in hair development.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FGF-22 (A96) polyclonal antibody detects endogenous levels of FGF-22 protein.

Bioworld Molecular Weight: ~ 20 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FGF 22 (A96) pAb at 1:500 dilution
Lane 1: HEK293T whole cell lysate (40ug)
Lane 2: MCF-7 whole cell lysate (40ug)
Lane 3: C6 whole cell lysate (40ug)
Lane 4: BV2 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of FGF 22 (A96) pAb in paraffin-embedded human lung carcinoma tissue at 1:50, showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of FGF 22 (A96) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50, showing secreted staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: Fibroblast growth factor 22; FGF-22; FGF22; UNQ2500/PRO5800

Immunogen: Synthetic peptide, corresponding to amino acids 64-112 of Human FGF 22.

Conjugate: Unconjugated

Modification: Unmodified