Product Description

FHIT (E116) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1126-50, BS1126-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49789

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200
IP: 1:50~1:200

Background: FHIT, a candidate tumor suppressor gene, contains the FRA3B common fragile site and is highly susceptible to carcinogen damage. The pattern of mutational inactivation seen with the FHIT gene is unique compared with other known tumor suppressors. FHIT gene structure and expression have been shown to be altered in esophageal, head, neck, lung, gastric, breast, and cervical carcinomas. It has been demonstrated that FHIT exon loss is associated with smoking duration or asbestos exposure. The FHIT protein is a member of the histidine triad (HIT) superfamily and functions as a dinucleoside 5’,5’’’-P1,P3-triphosphate hydrolase.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: FHIT (E116) polyclonal antibody detects endogenous levels of FHIT protein.

Bioworld Molecular Weight: ~ 17 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of FHIT (E116) pAb at 1:500 dilution
Lane 1: SK-OVCAR3 whole cell lysate (40ug)
Lane 2: The Kidney tissue lysate of Mouse (40ug)
Lane 3: MCF-7 whole cell lysate (40ug)
Lane 4: SGC7901 whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of FHIT (E116) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of MCF-7 cell lysate using FHIT (E116) polyclonal antibody (Sepharose Bead Conjugate) #BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) #BD0048 (Lane 4). Lane 1 is 30% input. The western blot was probed using FHIT (E116) #BS1126. “↑”（supernatant）;“↓(deposition）

Alternative Name: Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5',5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein; FHIT

Immunogen: Synthetic peptide, corresponding to amino acids 100-150 of Human FHIT.

Conjugate: Unconjugated

Modification: Unmodified