Product Description

Fibrillarin Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16628-50, BZ16628-100

Product: Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Swiss-Prot: P22087

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC-P, ICC/IF, IP, , FC

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 IP: 1/20 FC: 1/50-1/100

Background: Fibrillarin is a 2'-O-methyltransferase located in fibrillar regions and Cajal bodies of the nucleolus, where RNA transcription and pre-RNA processing take place. Fibrillarin associates with several other structual proteins as well as box C/D snoRNA to form a complex that functions in pre-rRNA processing, pre-rRNA methylation and ribosome assembly. This complex catalyzes site-specific 2'-O-ribose methylation of targeted nucleotides within the rRNA sequence. The sequence, structure and function of fibrillarin are highly conserved and fibrillarin gene expression is essential for early embryonic development.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 34 kDa; Observed MW: 34 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Fibrillarin in HepG2 lysates using Fibrillarin antibody., Immunohistochemistry analysis of paraffin-embedded Human testis using Fibrillarin antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunofluorescence analysis of Fibrillarin in Hela using Fibrillarin antibody.

Alternative Name: FIB; FLRN; Nop1; RNU3IP1

Immunogen: A synthesized peptide derived from human Fibrillarin

Conjugate: Unconjugated

Modification: Unmodified