Product Description

Filamin 1 (R2146) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1128-50, BS1128-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P21333

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200
IF: 1:50~1:200

Background: Caldesmon, Filamin 1, nebulin and villin are differentially expressed and regulated Actin binding proteins. Both muscular (CDh) and non-muscular (CDl) forms of caldesmon have been identified and each has been shown to bind to Actin as well as to calmodulin and Myosin. CDh is expressed predominantly on thin filaments in smooth muscle, whereas CDl is widely expressed in nonmuscle tissues and cells. Filamin 1, which is ubiquitously expressed and exists as a homodimer, functions to crosslink Actin to filaments. Nebulin is a large filamentous protein specific to muscle tissue that may function as a ruler for filament length. Several isoforms of nebulin, ranging from 700 to 900 kDa, are produced by alternative exon usage. Villin is Ca2+-regulated and is the major structural component of the brush border of absorptive cells.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Filamin 1 (R2146) polyclonal antibody detects endogenous levels of Filamin A protein.

Bioworld Molecular Weight: ~ 280 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Filamin 1 (R2146) polyclonal antibody at 1:500 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: Raw264.7 whole cell lysate
Lane 3: H9C2 whole cell lysate, Immunohistochemistry (IHC) analyzes of Filamin 1 (R2146) pAb in paraffin-embedded human breast carcinoma tissue at 1:100., IF image of BS1128 stained 3T3-L1 cells. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody Filamin 1 (R2146) pAb#BS1128 (1:100) at 5µg/ml overnight at +4°C. The secondary antibody (Green) was Goat anti-Rabbit IgG (H+L) -FITC#BS10950 used at a 1/200 dilution for 1h. Hoechst 33342 Staining Kit#BD5011 was used to stain the cell nuclei (blue).

Alternative Name: FilaminA; Filamin A; Filamin-A; FLN-A; Actin-binding protein 280; ABP-280; Alpha-filamin; Endothelial actin-binding protein; Filamin-1; Filamin1; Non-muscle filamin; FLNA; FLN; FLN1

Immunogen: Synthetic peptide, corresponding to amino acids 2100-2200 of Human Filamin 1.

Conjugate: Unconjugated

Modification: Unmodified