Fli-1 (R319) Polyclonal Antibody-BS1129

Fli-1 (R319) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1129-50, BS1129-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q01543

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Ets-1 is the prototype member of a family of genes identified on the basis of homology to the v-Ets oncogene isolated from the E26 erythroblastosis virus. This family of genes currently includes Ets-1, Ets-2, Erg-1–3, Elk-1, Elf-1, Elf-5, NERF, PU.1, PEA3, ERM, FEV, ER8l, Fli-1, TEL, Spi-B, ESE-1, ESE-3A, Net, ABT1 and ERF. Members of the Ets gene family exhibit varied patterns of tissue expression, and share a highly conserved carboxy-terminal domain containing a sequence related to the SV40 large T antigen nuclear localization signal sequence. This conserved domain is essential for Ets-1 binding to DNA and is likely to be responsible for the DNA binding activity of all members of the Ets gene family. Several of these proteins have been shown to recognize similar motifs in DNA that share a centrally located 5'-GGAA-3' element.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of Fli-1 (R319) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 50 kDa

Specificity: FLI1 (R319) polyclonal antibody detects endogenous levels of FLI1 protein.

Alternative Name: Friend leukemia integration 1 transcription factor; Proto-oncogene Fli-1; Transcription factor ERGB; FLI1

Immunogen: Synthetic peptide, corresponding to amino acids 300-350 of Human Fli-1.

Conjugate: Unconjugated

Modification: Unmodified