Product Description

FLI1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66007-50, MB66007-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Friend leukemia integration 1 (FLI1) transcription factor is an ETS domain-containing transcription factor that plays an important and highly conserved role in vertebrate development, particμlarly hematopoiesis, where it functions to activate transcription of genes that promote erythroblast proliferation. In mice, the Fli1 locus is a common retroviral insertion site for the Friend murine leukemia virus (F-MμlV), such that a majority of F-MμlV-induced erythroleukemias are associated with aberrant Fli1 expression. Notably in humans, aberrant FLI1 expression has also been linked to poor prognosis in acute myeloid leukemia. Also in humans, a t(11, 22)(q24, q12) chromosomal translocation has been described that generates a chimeric protein (EWS/FLI1) comprised of the amino-terminal transactivation domain of Ewing's sarcoma breakpoint region 1 (EWS) and the carboxy-terminal ETS domain of FLI1. The EWS/FLI1 fusion protein functions as a transcriptional activator that is reportedly responsible for >85% of the known cases of pediatric Ewing’s sarcoma, an aggressive bone and soft tissue tumor.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: Friend leukemia integration 1 transcription factor, Proto-oncogene Fli-1, Transcription factor ERGB

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human FLI1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of FLI1 protein.

Molecular Weight: ~ 50 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q01543

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only