Product Description

Flt-1 (phospho Tyr1333) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1485-50, ES1485-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the vascular endothelial growth factor receptor (VEGFR) family. VEGFR family members are receptor tyrosine kinases (RTKs) which contain an extracellular ligand-binding region with seven immunoglobulin (Ig)-like domains, a transmembrane segment, and a tyrosine kinase (TK) domain within the cytoplasmic domain. This protein binds to VEGFR-A, VEGFR-B and placental growth factor and plays an important role in angiogenesis and vasculogenesis. Expression of this receptor is found in vascular endothelial cells, placental trophoblast cells and peripheral blood monocytes. Multiple transcript variants encoding different isoforms have been found for this gene. Isoforms include a full-length transmembrane receptor isoform and shortened, soluble isoforms. The soluble isoforms are associated with the onset of pre-eclampsia.[provided by RefSeq, May 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: FLT1, FLT, FRT, VEGFR1, Vascular endothelial growth factor receptor 1, VEGFR-1, Fms-like tyrosine kinase 1, FLT-1, Tyrosine-protein kinase FRT, Tyrosine-protein kinase receptor FLT, FLT, Vascular permeability factor receptor

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human VEGFR1 around the phosphorylation site of Tyr1333. AA range:1289-1338

Isotype: IgG

Target: Flt-1

Specificity: Phospho-Flt-1 (Y1333) Polyclonal Antibody detects endogenous levels of Flt-1 protein only when phosphorylated at Y1333.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in normal lung, but also in placenta, liver, kidney, heart and brain tissues, Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes, Isoform 2 is strongly expressed in placenta, Isoform 3 is expressed in corneal epithelial cells (at protein level), Isoform 3 is expressed in vascular smooth muscle cells (VSMC),

Observed Band (KD): 151kD

Fields: MAPK signaling pathway, Ras signaling pathway, Rap1 signaling pathway, Calcium signaling pathway, HIF-1 signaling pathway, PI3K-Akt signaling pathway, Focal adhesion, Transcriptional misregulation in cancer, Rheumatoid arthritis

Function: Alternative Products: Additional isoforms seem to exist,Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Function: Receptor for VEGF, VEGFB and PGF. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. Isoform SFlt1 may have an inhibitory role in angiogenesis
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily
Similarity: Contains 1 protein kinase domain
Similarity: Contains 7 Ig-like C2-type (immunoglobulin-like) domains
Subunit: Interacts in vitro with various phosphotyrosine-binding proteins, including PLC-gammas, PTPN11, GRB2, CRK and NCK1
Tissue Specificity: Mostly in normal lung, but also

Subcellular Location: [Isoform 1]: Cell membrane, Single-pass type I membrane protein Endosome Autophosphorylation promotes ubiquitination and endocytosis, [Isoform 2]: Secreted, [Isoform 3]: Secreted, [Isoform 4]: Secreted, [Isoform 5]: Cytoplasm, [Isoform 6]: Cytoplasm, [Isoform 7]: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: FLT1

Protein Name: Vascular endothelial growth factor receptor 1

Mouse Gene ID: 14254

Mouse SwissProt Number: P35969

Rat Gene ID: 54251

Rat SwissProt Number: P53767

Research Use Only