Product Description

Flt3 (phospho Tyr599) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5320-50, ES5320-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015],

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Applications: WB, ELISA

Alternate Names: FLT3, CD135, FLK2, STK1, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Stem cell tyrosine kinase 1, STK-1, CD antigen CD135

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr599. AA range:565-614

Isotype: IgG

Target: Flt3

Specificity: Phospho-Flt3 (Y599) Polyclonal Antibody detects endogenous levels of Flt3 protein only when phosphorylated at Y599.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in bone marrow, in hematopoietic stem cells, in myeloid progenitor cells and in granulocyte/macrophage progenitor cells (at protein level), Detected in bone marrow, liver, thymus, spleen and lymph node, and at low levels in kidney and pancreas, Highly expressed in T-cell leukemia,

Observed Band (KD): 160kD

Fields: MAPK signaling pathway, Ras signaling pathway, PI3K-Akt signaling pathway, Hematopoietic cell lineage, Pathways in cancer, Transcriptional misregulation in cancer, Acute myeloid leukemia, Central carbon metabolism in cancer

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Function: Receptor for the FL cytokine. Has a tyrosine-protein kinase activity
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily
Similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain
Similarity: Contains 1 protein kinase domain
Subunit: Interacts with FIZ1 following ligand activation
Tissue Specificity: Bone marrow cells

Subcellular Location: Membrane, Single-pass type I membrane protein Endoplasmic reticulum lumen Constitutively activated mutant forms with internal tandem duplications are less efficiently transported to the cell surface and a significant proportion is retained in an immature form in the endoplasmic reticulum lumen The activated kinase is rapidly targeted for degradation

Storage and Stability: -20°C/1 year

Gene Name: FLT3

Protein Name: Receptor-type tyrosine-protein kinase FLT3

Mouse Gene ID: 14255

Mouse SwissProt Number: Q00342

Research Use Only