Product Description

FoxO1A Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2365-50, ES2365-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: FOXO1, FKHR, FOXO1A, Forkhead box protein O1, Forkhead box protein O1A, Forkhead in rhabdomyosarcoma

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human FOXO1A. AA range:295-344

Isotype: IgG

Target: FoxO1

Specificity: FoxO1A Polyclonal Antibody detects endogenous levels of FoxO1A protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous,

Observed Band (KD): 70kD

Fields: FoxO signaling pathway, AMPK signaling pathway, Longevity regulating pathway, Longevity regulating pathway - multiple species, Cellular senescence, Insulin signaling pathway, Thyroid hormone signaling pathway, Glucagon signaling pathway, Insulin resistance, AGE-RAGE signaling pathway in diabetic complications, Alcoholic liver disease, Shigellosis, Human papillomavirus infection, Pathways in cancer, Transcriptional misregulation in cancer, Prostate cancer

Function: Disease: Chromosomal aberrations involving FOXO1 are a cause of rhabdomyosarcoma 2 (RMS2) [MIM: 268220], also known as alveolar rhabdomyosarcoma. Translocation (2, 13)(q35, q14) with PAX3, translocation t(1, 13)(p36, q14) with PAX7. The resulting protein is a transcriptional activator
Function: Transcription factor
PTM: Phosphorylated by AKT1, insulin-induced (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR
Similarity: Contains 1 fork-head DNA-binding domain
Subcellular Location: Shuttles betw

Subcellular Location: Cytoplasm Nucleus Shuttles between the cytoplasm and nucleus Largely nuclear in unstimulated cells (PubMed:11311120, PubMed:12228231, PubMed:19221179, PubMed:21245099, PubMed:20543840, PubMed:25009184) In osteoblasts, colocalizes with ATF4 and RUNX2 in the nucleus (By similarity) Serum deprivation increases localization to the nucleus, leading to activate expression of SOX9 and subsequent chondrogenesis (By similarity) Insulin-induced phosphorylation at Ser-256 by PKB/AKT1 leads, via stimulation of Thr-24 phosphorylation, to binding of 14-3-3 proteins and nuclear export to the cytoplasm where it is degraded by the ubiquitin-proteosomal pathway (PubMed:11237865, PubMed:12228231) Phosphorylation at Ser-249 by CDK1 disrupts binding of 14-3-3 proteins and promotes nuclear accumulation

Storage and Stability: -20°C/1 year

Gene Name: FOXO1

Protein Name: Forkhead box protein O1

Mouse Gene ID: 56458

Mouse SwissProt Number: Q9R1E0

Rat Gene ID: 84482

Rat SwissProt Number: G3V7R4

Research Use Only