FUBP3 Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB67176-50, MB67176-100 Product: Mouse IgG1. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide. Swiss-Prot: Q96I24 Host: Mouse Reactivity: Human Applications: WB, IF/ICC All Applications: WB (1/500 - 1/1000), IF/ICC (1/10 - 1/50) Background: Activation of FUSE, the far-upstream element, is required for the proper expression of the mammalian gene c-Myc in undifferentiated cells. The binding of FBP (FUSE-binding protein or far upstream element binding protein) to FUSE is necessary for c-Myc expression, indicating that FBP functions as a growth-dependent regulator of c-Myc expression. Isolated from proliferating HL60 cells, FBP, FBP2, and FBP3 comprise a family of single-stranded DNAbinding proteins that specifically bind to FUSE elements. The FBP transcription factors share a conserved central DNA-binding domain and show significant homology in their carboxyl-terminal activation domains. Expression of FBP is detected in undifferentiated cells and is substantially decreased following cellular differentiation. Purification and Purity: This antibody is purified through a protein G column. Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of FUBP3 protein. Bioworld Molecular Weight: ~ 62 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Western blot analysis of FUBP3 expression in Hela (A), U251 (B) whole cell lysates., Immunofluorescent analysis of FUBP3 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue). Alternative Name: FBP3; Far upstream element-binding protein 3; FUSE-binding protein 3 Immunogen: Recombinant fusion protein of human FUBP3. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified