Product Description

GAD-67 (A492) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1135-50, BS1135-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q99259

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: GAD-65 and GAD-67 are members of the group II decarboxylase family of proteins and are responsible for catalyzing the rate limiting step in the production of GABA (γ-aminobutyric acid) from L-glutamic acid. Although both GADs are found in the brain, GAD-65 localizes to synaptic vesicle membranes in nerve terminals, while GAD-67 is distributed throughout the cell. GAD-67 is responsible for the basal levels of GABA synthesis. In the case of a heightened demand for GABA in neurotransmission, GAD-65 will transiently activate to assist in GABA production. The loss of GAD-65 is detrimental and can impair GABA neurotransmission, however the loss of GAD-67 is lethal. Due to alternative splicing, two isoforms exist for GAD-67: the predominant GAD-67 form and the minor GAD-25 form.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of GAD-67 (A492) pAb in paraffin-embedded human breast carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 67 kDa

Specificity: GAD67 (A492) polyclonal antibody detects endogenous levels of GAD67 protein.

Alternative Name: Glutamate decarboxylase 1; 67 kDa glutamic acid decarboxylase; GAD 67; Glutamate decarboxylase 67 kDa isoform; GAD1; GAD; GAD67; GAD-1; GAD 1

Immunogen: Synthetic peptide, corresponding to amino acids 460-514 of Human GAD-67.

Conjugate: Unconjugated

Modification: Unmodified