GAK (Q129) Polyclonal Antibody-BS1871

GAK (Q129) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1871-50, BS1871-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O14976

Host: Rabbit

Reactivity: Human, Mouse

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Cyclin D1 accumulates during G1 and associates with Cdk2, Cdk4 and Cdk5. Cyclin E and Cdk2 interact during the G1 to S transition. Cyclin G contains a typical N terminal cyclin box and a carboxy terminal domain sequence homologous to the tyrosine phosphorylation site of the epidermal growth factor receptor). Cyclin G expression is induced within 3 hours after growth stimulation and remains elevated with no apparent cell cycle dependency. A serine/threonine kinase, designated GAK for cyclin G associated kinase, has been identified. GAK has been shown to bind directly to cyclin G and to co-immunoprecipitate with Cdk5, which also associates with cyclin G.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of GAK (Q129) pAb in paraffin-embedded human kidney carcinoma tissue at 1:50. showing Cytoplasm staining. Negative control (the right) Using PBS instead of primary antibody,Secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Bioworld Molecular Weight: ~ 143 kDa

Specificity: GAK (Q129) polyclonal antibody detects endogenous levels of GAK protein.

Alternative Name: Cyclin-G-associated kinase

Immunogen: Synthetic peptide, corresponding to amino acids 101-150 of Human GAK.

Conjugate: Unconjugated

Modification: Unmodified