Product Description

GATA-4 (phospho Ser262) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5482-50, ES5482-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the GATA family of zinc-finger transcription factors. Members of this family recognize the GATA motif which is present in the promoters of many genes. This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function, and is necessary for normal testicular development. Mutations in this gene have been associated with cardiac septal defects. Additionally, alterations in gene expression have been associated with several cancer types. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IF, ELISA

Alternate Names: GATA4, Transcription factor GATA-4, GATA-binding factor 4

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human GATA4 around the phosphorylation site of Ser262. AA range:228-277

Isotype: IgG

Target: GATA-4

Specificity: Phospho-GATA-4 (S262) Polyclonal Antibody detects endogenous levels of GATA-4 protein only when phosphorylated at S262.

Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Heart, Lung,

Observed Band (KD): 48kD

Fields: cGMP-PKG signaling pathway, Cellular senescence, Tight junction, Thyroid hormone signaling pathway

Function: Disease: Defects in GATA4 are the cause of atrial septal defect type 2 (ASD2) [MIM: 607941]. ASD2 is a congenital heart malformation characterized by incomplete closure of the wall between the atria resulting in blood flow from the left to the right atria. ASD2 patients show other heart abnormalities including ventricular and atrioventricular septal defects, pulmonary valve thickening or insufficiency of the cardiac valves. ASD2 is not associated with defects in the cardiac conduction system or non-cardiac abnormalities
Function: Transcriptional activator. Binds to the consensus sequence 5'-AGATAG-3'. Acts as a transcriptional activator of ANF in cooperation with NKX2-5
Similarity: Contains 2 GATA-type zinc fingers
Subunit: Interacts with ZNF260 (By similarity). Interacts with the homeobox domain of NKX2-5 through its C-terminal zinc finger. Also interacts with JARID2 which represses its a

Subcellular Location: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: GATA4

Protein Name: Transcription factor GATA-4

Mouse Gene ID: 14463

Mouse SwissProt Number: Q08369

Rat Gene ID: 54254

Rat SwissProt Number: P46152

Research Use Only