GFP-tag (3A10) Monoclonal Antibody-HRP-AP0675MH

GFP-tag (3A10) Monoclonal Antibody-HRP

Sizes: 50µl, 100µl

Catalogue Numbers: AP0675MH-50, AP0675MH-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Mouse

Reactivity: All

Applications: WB,

All Applications: WB:1:5000~1:10000

Background: Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

Purification and Purity: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GFP-tag (3A10) mAb-HRP detects over-expressed or recombinant proteins containing the GFP epitope tag.

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of over-expressed GFP-tagged protein in HEK293T cell lysate, the antibody dilutions are 1:5000 (Lane 1) and 1:10000 (Lane 2). Each Lane was loaded with 30 μg of cell lysate., Western blot (WB) analysis of GFP-tag (3A10) mAb-HRP at 1:2000/1:5000/1:10000 dilution
Lane 1: HEK293T whole cell lysate
Lane 2: HEK293T whole cell lysate,transfected with pEGFP-C3-ATG3.
Lane 3: HEK293T whole cell lysate,transfected with pEGFP-C3-ATG12.

Alternative Name: Green fluorescent protein

Immunogen: Synthetic peptide GFP-tag.

Conjugate: HRP

Modification: Unmodified