Product Description

GFP-tag (3A10) Monoclonal Antibody-HRP

Sizes: 50μl, 100μl

Catalogue Numbers: AP0675MH-50, AP0675MH-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Green fluorescent protein (GFP) is a 27 a protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

Category: Primary Antibody

Reactivity: Transfected

Host: Mouse

Applications: WB

Alternate Names: Green fluorescent protein

Clonality: Monoclonal

Conjugate: HRP

Modification: Unmodification

Immunogen: Synthetic peptide GFP-tag.

Dilution: WB:1:5000~1:10000

Purification: Antibodies were immunoaffinity purified using the peptide conjugated to a solid-phase support and conjugated to horseradish peroxidase. The antibody was affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: GFP-tag (3A10) mAb-HRP detects over-expressed or recombinant proteins containing the GFP epitope tag.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only