GH1 Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66072-50, MB66072-100 Product: Mouse IgG. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: P01241 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: Pituitary growth hormone (GH), also designated somatotropin, plays a crucial role in stimulating and controlling the growth, metabolism and differentiation of many mammalian cell types by modulating the synthesis of multiple mRNA species. These effects are mediated by the binding of GH to its membranebound receptor, GHR, and involve a phosphorylation cascade that results in the modulation of numerous signaling pathways. GH is secreted in a pulsatile pattern which is tightly controlled by the interplay of GH-releasing hormone (GHRH) and somatostatin (SRIF). GHRH and SRIF are the primary hypothalamic factors that determine GH secretion from the somatotroph and regulate GH synthesis and secretory reserve. GH output is also highly sensitive to feedback control by GH itself, as well as by Insulin-like growth factor I. GH is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human growth hormone contains 191 amino acid residues with two disulfide bridges. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Specificity: Recognizes endogenous levels of GH1 protein. Bioworld Molecular Weight: ~ 21 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of GH1 staining in human hyperprolaceinemia formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunohistochemical analysis of GH1 staining in human pituitary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Alternative Name: Somatotropin; Growth hormone; GH; GH-N; Growth hormone 1; Pituitary growth hormone Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human GH1. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified