Product Description

Glucokinase (GCK) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS8515-50, BS8515-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene resμlts in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insμlin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsμlinemic hypoglycemia of infancy (PHHI).

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: GCK, FGQTL3, GK, GLK, HHF3, HK4, HKIV, HXKP, LGLK, MODY2

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 250-465 of human Glucokinase (Glucokinase (GCK)) (NP_000153.1).

Dilution: WB: 1:500 - 1:2000
IHC: 1:50 - 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Glucokinase (GCK) polyclonal antibody detects endogenous levels of Glucokinase (GCK) protein.

Molecular Weight: ~ 80, 55 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P35557

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only