Product Description

Glucose Uptake Fluorometric Assay Kit

Sizes: 96T, 500 Assays

Catalogue Numbers: E-BC-F041-96, E-BC-F041-500

Citations, Manuals and MSDS Available upon request.

Research Areas: Metabolic Diseases;Glycolysis and Lipid Metabolism

Result Type: Quantitative

Detection Instrument: Fluorescence microplate reader

Tested Sample Types: cells

Assay Time: 100 min

Detection Type: Fluorescence method

Sensitivity: 0.02 nmol/μL

Detection Range: 0.02-0.3 nmol/μL

Test Principle: 2-DG is up-taken by the cells, converted to 2-DG-6P, which is catalyzed by glucose dehydrogenase to produce 6PDG. Meanwhile, NADP+ is converted to NADPH. The generated NADPH converts the probe into fluorescent substances under the action of myocardial yellow transferase. The glucose uptake can be calculated by measuring the fluorescence intensity at the excitation wavelength of 530 nm and the emission wavelength of 590 nm.

Storage: This product can be stored at -20°C for 12 months with shading light.

Shipping: Ice bag