Product Description

GLUT4 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66704-50, MB66704-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: A group of related glucose transporters (Glut1-5 and 7) mediate the facilitated diffusion of glucose in nonepithelial mammalian tissues. Within insμlin-responsive tissues such as muscle and fat, Glut1 contributes to basal glucose uptake while Glut4 is responsible for insμlin-stimμlated glucose transport. Glut4 is a 12-transmembrane domain protein that facilitates glucose transport in the direction of the glucose gradient. This transporter localizes to intracellμlar organelles (endosomes) in unstimμlated cells and translocates to the cell surface following insμlin stimμlation. Translocation of Glut4 is dependent on Akt, which may act by phosphorylating AS160, a RabGAP protein involved in membrane trafficking.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB, IHC, IF/ICC, FC

Alternate Names: GLUT4, Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insμlin-responsive, GLUT-4

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fragment of human GLUT4 expressed in E. Coli. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/500), IF/ICC (1/100 - 1/500), FC (1/100 - 1/200)

Purification: The antibody was purified by protein G.

Specificity: Recognizes endogenous levels of GLUT4 protein.

Molecular Weight: ~ 55 kDa

Pathway: AMPK Signaling Pathway, Insμlin &Glucose Signaling, Warburg Effect.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P14672

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only