Product Description

GlyRβ Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5566-50, ES5566-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes the beta subunit of the glycine receptor, which is a pentamer composed of alpha and beta subunits. The receptor functions as a neurotransmitter-gated ion channel, which produces hyperpolarization via increased chloride conductance due to the binding of glycine to the receptor. Mutations in this gene cause startle disease, also known as hereditary hyperekplexia or congenital stiff-person syndrome, a disease characterized by muscular rigidity. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: GLRB, Glycine receptor subunit beta, Glycine receptor 58 kDa subunit

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human GLRB. AA range:211-260

Isotype: IgG

Target: GlyRβ

Specificity: GlyRβ Polyclonal Antibody detects endogenous levels of GlyRβ protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Hippocampus,

Observed Band (KD): 56kD

Fields: Neuroactive ligand-receptor interaction

Function: Disease: Defects in GLRB are a cause of startle disease (STHE) [MIM: 149400], also known as hereditary hyperekplexia or congenital stiff-person syndrome. STHE is a genetically heterogeneous neurologic disorder characterized by muscular rigidity of central nervous system origin, particularly in the neonatal period, and by an exaggerated startle response to unexpected acoustic or tactile stimuli. Inheritance can be autosomal dominant or recessive
Function: The glycine receptor is a neurotransmitter-gated ion channel. Binding of glycine to its receptor increases the chloride conductance and thus produces hyperpolarization (inhibition of neuronal firing)
Similarity: Belongs to the ligand-gated ionic channel (TC 1.A.9) family
Subunit: Pentamer composed of alpha and beta subunits. Interacts with GPHN

Subcellular Location: Cell junction, synapse, postsynaptic cell membrane, Multi-pass membrane protein Cell junction, synapse Cell projection, dendrite Cell membrane, Multi-pass membrane protein Cytoplasm Retained in the cytoplasm upon heterologous expression by itself Coexpression with GPHN promotes expression at the cell membrane (PubMed:12684523) Coexpression with GLRA1, GLRA2 or GLRA3 promotes expression at the cell membrane

Storage and Stability: -20°C/1 year

Gene Name: GLRB

Protein Name: Glycine receptor subunit beta

Mouse Gene ID: 14658

Mouse SwissProt Number: P48168

Rat Gene ID: 25456

Rat SwissProt Number: P20781

Research Use Only