GlyRS Monoclonal Antibody-MB66982

GlyRS Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66982-50, MB66982-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P41250

Host: Mouse

Reactivity: Human

Applications: WB, IF/ICC, FC

All Applications: WB (1/1000 - 1/2000), IF/ICC (1/10 - 1/50)

Background: Catalyzes the ATP-dependent ligation of glycine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (Gly-AMP) .Also produces diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs. Thereby, may play a special role in Ap4A homeostasis .

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GlyRS protein.

Bioworld Molecular Weight: ~ 85 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GlyRS expression in HT1080 (A), Jurkat (B), Ramos (C) whole cell lysates., Immunofluorescent analysis of GlyRS staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF555 was used to stain the cytoplasm (red). DAPI was used to stain the cell nuclei (blue).

Alternative Name: Glycine--tRNA ligase; Diadenosine tetraphosphate synthetase; AP-4-A synthetase; Glycyl-tRNA synthetase; GlyRS

Immunogen: Recombinant fusion protein of human GlyRS. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified