GNAS Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A5546-20, A5546-100
Citations, Manuals and MSDS Available upon request.
Background: This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons. Some transcripts contain a differentially methylated region (DMR) at their 5' exons, and this DMR is commonly found in imprinted genes and correlates with transcript expression. An antisense transcript is produced from an overlapping locus on the opposite strand. One of the transcripts produced from this locus, and the antisense transcript, are paternally expressed noncoding RNAs, and may regulate imprinting in this region. In addition, one of the transcripts contains a second overlapping ORF, which encodes a structurally unrelated protein - Alex. Alternative splicing of downstream exons is also observed, which results in different forms of the stimulatory G-protein alpha subunit, a key element of the classical signal transduction pathway linking receptor-ligand interactions with the activation of adenylyl cyclase and a variety of cellular reponses. Multiple transcript variants encoding different isoforms have been found for this gene. Mutations in this gene result in pseudohypoparathyroidism type 1a, pseudohypoparathyroidism type 1b, Albright hereditary osteodystrophy, pseudopseudohypoparathyroidism, McCune-Albright syndrome, progressive osseus heteroplasia, polyostotic fibrous dysplasia of bone, and some pituitary tumors.
Category: Polyclonal Antibodies
Applications: WB, IF/ICC, IP, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 28kDa/44kDa/45kDa/77kDa/109kDa/111kDa
Observed Molecular Weight: 50kDa
Immunogen: Synthetic peptide .
Species: Human
GeneID: 2778
SWISS: O95467/P63092/P84996/Q5JWF2
Alternate Names: AHO; GSA; GSP; POH; GPSA; NESP; SCG6; SgVI; GNAS1; PITA3; C20orf45; GNAS
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:500 - 1:2000 IF/ICC, 1:50 - 1:200 IP, 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Cancer, Signal Transduction, G protein signaling, G-Protein-Coupled Receptors Signaling to MAPK Erk, Endocrine Metabolism, Neuroscience, Neurodegenerative Diseases, Dopamine Signaling in Parkinson's Disease.
NCBI Alias: GNAS
Research Use Only