Product Description

GPNMB Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11148-50, ES11148-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: glycoprotein nmb(GPNMB) Homo sapiens The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: GPNMB,

Specificity: GPNMB Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Widely expressed, but very low expression, if any, in the brain (PubMed:12609765, PubMed:16609006). Expressed in the epidermis with higher levels in melanocytes compared with keratinocytes and Langerhans cells (at protein level) (PubMed:29336782). Expressed in peripheral blood, but not bone marrow mononuclear cells (PubMed:12609765). Expressed in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level) (PubMed:16489096). May be overexpressed in various cancers, including melanoma and glioblastoma multiforme (PubMed:7814155, PubMed:16489096, PubMed:16609006).

Observed Band (KD): 62kD

Function: Developmental Stage: Expression in poorly metastatic melanoma cell lines; no expression in highly metastatic melanoma cell lines. Could be a melanogenic enzyme. Similarity: Belongs to the Pmel-17/NMB family. Similarity: Contains 1 PKD domain. Subcellular Location: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Tissue Specificity: Not restricted to the melanocytic lineage.

Subcellular Location: Cell membrane, Single-pass type I membrane protein Melanosome membrane, Single-pass type I membrane protein Early endosome membrane, Single-pass type I membrane protein Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Storage and Stability: -20°C/1 year

Gene Name: GPNMB, HGFIN, NMB, UNQ1725/PRO9925

Protein Name: Transmembrane glycoprotein NMB, Transmembrane glycoprotein HGFIN

Human Gene ID: 10457

Human SwissProt Number: Q14956

Mouse SwissProt Number: Q99P91

Rat SwissProt Number: Q6P7C7

Research Use Only