Product Description

GPR152 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS67253-50, BS67253-100

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: Q8TDT2

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IF/IC

All Applications: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Background: GPR152 is one of nine new members of the Rhodopsin family of human G protein-coupled receptors (GPCRs), and is an Orphan-U GPCR with an unknown ligand. Members of the GPCR superfamily participate in a variety of physiological functions and are major targets of pharmaceutical drugs.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 214% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of GPR152 protein.

Bioworld Molecular Weight: ~ 50 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GPR152 expression in MCF7 (A), HCT116 (B), SGC7901 (C), mouse spleen (D), rat spleen (E), rat brain (F) whole cell lysates., Immunofluorescent analysis of GPR152 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Alternative Name: PGR5; Probable G-protein coupled receptor 152; G-protein coupled receptor PGR5

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human GPR152. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified