GPR30 Polyclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: BS65862-50, BS65862-100 Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Swiss-Prot: Q99527 Host: Rabbit Reactivity: Human, Mouse, Rat, Dog, Applications: WB, IHC-P All Applications: WB=1:500-2000 IHC-P=1:100-500 Background: G protein-coupled receptors (GPRs, or GPCRs) contain 7 hydrophobic transmembrane domains embedded in hydrophilic intra- and extracellular loops and transduce a variety of hormone, endogenous peptide, and neurotransmitter signals into intracellular effects via G proteins. GRP30 is a member of this family and is an orphan receptor. GPR30 expression has been reported in brain, breast carcinoma, blood, bone marrow, CNS, heart, liver, lung, lymph node, placenta, and spleen. In brain, GPR30 is expressed as a 2.8 kb transcript in basal forebrain, frontal cortex, thalamus, hippocampus, caudate and putamen. However, unlike other known G protein-coupled receptors, GPR30 localizes to the endoplasmic reticulum, where it specifically binds estrogen and estrogen derivatives. Purification and Purity: affinity purified by Protein A Storage and Stability: Store at 4°C short term. Aliquot and store at -35°C long term. Avoid freeze-thaw cycles. Specificity: GPR30 Polyclonal Antibody detects endogenous levels of GPR30 protein. Bioworld Molecular Weight: ~55 kDa Note: For research use only, not for use in diagnostic procedure. Extra Notes: Primary: Anti-GPR30 at 1/300 dilution, Primary: Anti-GPR30 at 1/1000 dilution, Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR30) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C Alternative Name: G Protein Coupled Receptor 30; G-protein coupled receptor 30; G-protein coupled estrogen receptor 1; Membrane estrogen receptor; mER; Chemokine receptor-like 2; IL8-related receptor DRY12; Flow-induced endothelial G-protein coupled receptor 1; FEG-1; Lymphocyte-derived G-protein coupled receptor; LYGPR; GPCR-BR; CEPR; CMKRL2; DRY12; GPER. Immunogen: KLH conjugated synthetic peptide derived from human GPR30:251-350/375 Conjugate: Unconjugated Modification: Unmodified