Product Description

GPR91 (A135) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2961-50, BS2961-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9BXA5

Host: Rabbit

Reactivity: Human

Applications: WB, IF

All Applications: WB: 1:500~1:1000
IF: 1:50~1:200

Background: GPR91 (formerly known as P2U2) is a G protein-coupled, dicarboxylic acid succinate receptor. It has a high level of expression in the kidney, predominantly in the proximal tubules, and localizes to the plasma membrane. It has also been found at low levels in the liver and the spleen. GPR91 functions as a citric acid cycle intermediate succinate receptor. Two signaling pathways result from GPR91 activation, the pertussis-toxin-sensitive Gi/Go pathway and the pertussis-toxin-insensitive Gq pathway. Four amino acid residues are necessary for GPR91 activation by succinate: Arg 99, His 103, Arg 252 and Arg 281. GPR91 plays an important role in the succinate-induced hypertensive effect and may be involved in renovascular hypertension, a disease linked to
diabetes, renal failure and atherosclerosis.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GPR91 (A135) polyclonal antibody detects endogenous levels of GPR91 protein.

Bioworld Molecular Weight: ~ 38 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of GPR91 expression in H1792 (A), SHSY5Y (B)whole cell lysates., Immunofluorescence analysis of MCF-7 cells using GPR91 pAb at dilution of 1:50.

Alternative Name: G-protein coupled receptor 91; P2Y purinoceptor 1-like; SUCNR1; GPR91

Immunogen: Synthetic peptide, corresponding to Human GPR91.

Conjugate: Unconjugated

Modification: Unmodified