Product Description

GRK 2 (phospho Ser685) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4932-50, ES4932-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: ADRBK1, BARK, BARK1, GRK2, Beta-adrenergic receptor kinase 1, Beta-ARK-1, G-protein coupled receptor kinase 2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human GRK2 around the phosphorylation site of Ser685. AA range:640-689

Isotype: IgG

Target: GRK 2

Specificity: Phospho-GRK 2 (S685) Polyclonal Antibody detects endogenous levels of GRK 2 protein only when phosphorylated at S685.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in peripheral blood leukocytes,

Observed Band (KD): 80kD

Fields: Chemokine signaling pathway, Endocytosis, Hedgehog signaling pathway, Glutamatergic synapse, Olfactory transduction, Morphine addiction

Function: Catalytic Activity: ATP + [beta-adrenergic receptor] = ADP + [beta-adrenergic receptor] phosphate
Catalytic Activity: ATP + a protein = ADP + a phosphoprotein
Function: Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them
Online Information: Beta adrenergic receptor kinase entry,Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily
Similarity: Contains 1 AGC-kinase C-terminal domain
Similarity: Contains 1 PH domain
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 RGS domain
Subunit: Interacts with GIT1 (By similarity). Interacts with, and phosphorylates chemokine-stimulated CCR5
Tissue Specificity: Expressed in peripheral blood leukocytes

Subcellular Location: Cytoplasm Cell membrane Cell junction, synapse, postsynapse Cell junction, synapse, presynapse

Storage and Stability: -20°C/1 year

Gene Name: ADRBK1

Protein Name: Beta-adrenergic receptor kinase 1

Mouse SwissProt Number: Q99MK8

Rat Gene ID: 25238

Rat SwissProt Number: P26817

Research Use Only