Product Description

GRP78 (P641) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1154-50, BS1154-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P11021

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF, IP,

All Applications: WB: 1:500~1:1000
IHC/IF: 1:50~1:200
IP: 1:10~1:100

Background: The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: GRP78 (P641) polyclonal antibody detects endogenous levels of GRP78 protein.

Bioworld Molecular Weight: ~ 78 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of GRP78 (P641) polyclonal antibody at 1:500 dilution
Lane 1: L02 whole cell lysate (40ug)
Lane 2: HCT116 whole cell lysate (40ug)Lane 3: HCT116 whole cell lysate (40ug)
Lane 3: AML-12 whole cell lysate (40ug)
Lane 4: PC12 whole cell lysate (40ug)Lane 3: HCT116 whole cell lysate (40ug)
Lane 5: HEK293T whole cell lysate (40ug), Immunohistochemistry (IHC) analyzes of GRP78 (P641) pAbb in paraffin-embedded human breast carcinoma tissue at 1:100., Immunoprecipitation of L02 whole cell lysate using GRP78 (P641) pAb (Sepharose Bead Conjugate) #BD0047(Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0047 (Lane 4). Lane 1 is 20% input. The western blot was probed using GRP78 (P641) pAb #BS11542. “↑”（supernatant); “↓”（deposition）

Alternative Name: 78 kDa glucose-regulated protein; GRP-78; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; Heat shock 70 kDa protein 5; Immunoglobulin heavy chain-binding protein; BiP; HSPA5; GRP78

Immunogen: Synthetic peptide, corresponding to amino acids 611-660 of Human GRP78.

Conjugate: Unconjugated

Modification: Unmodified