Product Description

Hck (phospho Tyr410) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES5721-50, ES5721-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the Src family of tyrosine kinases. This protein is primarily hemopoietic, particularly in cells of the myeloid and B-lymphoid lineages. It may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Multiple isoforms with different subcellular distributions are produced due to both alternative splicing and the use of alternative translation initiation codons, including a non-AUG (CUG) codon. [provided by RefSeq, Feb 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: IHC, IF, ELISA

Alternate Names: HCK, Tyrosine-protein kinase HCK, Hematopoietic cell kinase, Hemopoietic cell kinase, p59-HCK/p60-HCK, p59Hck, p61Hck

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human HCK around the phosphorylation site of Tyr410. AA range:381-430

Isotype: IgG

Target: Hck

Specificity: Phospho-Hck (Y410) Polyclonal Antibody detects endogenous levels of Hck protein only when phosphorylated at Y410.

Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Detected in monocytes and neutrophils (at protein level), Expressed predominantly in cells of the myeloid and B-lymphoid lineages, Highly expressed in granulocytes, Detected in tonsil,

Molecular Weight (Da): 60kD

Fields: Chemokine signaling pathway, Fc gamma R-mediated phagocytosis, Kaposi sarcoma-associated herpesvirus infection

Function: Catalytic Activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Domain: The SH3 domain mediates binding to HIV-1 Nef
Function: May serve as part of a signaling pathway coupling the Fc receptor to the activation of the respiratory burst. May also contribute to neutrophil migration and may regulate the degranulation process of neutrophils
PTM: Isoform p59-HCK contains a N-myristoyl glycine at position 3 (By similarity). Isoform p59-HCK contains a S-palmitoyl cysteine at position 3
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily
Similarity: Contains 1 protein kinase domain
Similarity: Contains 1 SH2 domain
Similarity: Contains 1 SH3 domain
Subunit: May interact (via SH3 domain) with HIV-1 Nef and Vif. This interaction would stimulates its tyrosine-kinase activity. Interacts (via SH3 domain) with HEV ORF3 protein
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Subcellular Location: [Isoform 1]: Lysosome Membrane, Lipid-anchor Cell projection, podosome membrane, Lipid-anchor Cytoplasm, cytosol Associated with specialized secretory lysosomes called azurophil granules At least half of this isoform is found in the cytoplasm, some of this fraction is myristoylated, [Isoform 2]: Cell membrane, Lipid-anchor Membrane, caveola, Lipid-anchor Cell junction, focal adhesion Cytoplasm, cytoskeleton Golgi apparatus Cytoplasmic vesicle Lysosome Nucleus 20% of this isoform is associated with caveolae Localization at the cell membrane and at caveolae requires palmitoylation at Cys-3 Colocalizes with the actin cytoskeleton at focal adhesions, Cytoplasmic vesicle, secretory vesicle Cytoplasm, cytosol

Storage and Stability: -20°C/1 year

Gene Name: HCK

Protein Name: Tyrosine-protein kinase HCK

Mouse Gene ID: 15162

Mouse SwissProt Number: P08103

Rat Gene ID: 25734

Rat SwissProt Number: P50545

Research Use Only