Product Description

HDAC1 (phospho-S421) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4693-50, BS4693-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Acetylation of lysine residues in the amino-terminal tail domain of histone resμlts in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated mammalian RPD3) and HDAC3, all of which are related to the yeast transcriptional regμlator Rpd3p, have been identified as histone deacetylases.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Histone deacetylase 1, HD1, RPD3L1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human HDAC1 around the phosphorylation site of Serine 421.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-HDAC1 (S421) polyclonal antibody detects endogenous levels of HDAC1 protein only when phosphorylated at Ser421.

Molecular Weight: ~ 55 kDa

Pathway: Notch Signaling, Nuclear Receptor Signaling, Cell Cycle Control G1 S Checkpoint, DNA Methylation, Epigenetic Writers and Erasers of Histone H3, Epigenetic Writers and Erasers of Histones H2A H2B and H4,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q13547

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only