HES-6 (A6) Polyclonal Antibody-BS2344

HES-6 (A6) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2344-50, BS2344-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96HZ4

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: The HES family members form a complex with TLE, the mammalian homologue of Groucho, and this interaction is mediated by the carboxy terminal WRPW motif of the HES proteins. The HES/TLE complex functions by directly binding to DNA, instead of interfering with activator proteins. Most HES family members, including HES1 and HES5, preferentially bind to the N box (CACNAG) as opposed to the E box (CANNTG). HES2 binds to both N and E box sites, while HES6 does not bind DNA. Rather, HES6 inhibits HES1 activity, thereby promoting transcription. HES1 and HES2 are expressed in a variety of adult and embryonic tissues. HES3 is expressed exclusively in cerebellar Purkinje cells, and HES5 is found solely in the nervous system. HES6 is produced in brain as well as in the limb buds of developing embryos.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of HES-6 (A6) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 30 kDa

Specificity: HES-6 (A6) polyclonal antibody detects endogenous levels of HES-6 protein.

Alternative Name: Transcription cofactor HES-6; C-HAIRY1; Class B basic helix-loop-helix protein 41; bHLHb41; Hairy and enhancer of split 6; BHLHB41; HES6

Immunogen: Synthetic peptide, corresponding to the N-terminal of Human HES-6.

Conjugate: Unconjugated

Modification: Unmodified