Product Description

Hexim1 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4522-50, ES4522-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Expression of this gene is induced by hexamethylene-bis-acetamide in vascular smooth muscle cells. This gene has no introns. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: HEXIM1, CLP1, EDG1, HIS1, MAQ1, Protein HEXIM1, Cardiac lineage protein 1, Estrogen down-regulated gene 1 protein, Hexamethylene bis-acetamide-inducible protein 1, Menage a quatre protein 1

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human HEXIM1. AA range:181-230

Isotype: IgG

Target: Hexim1

Specificity: Hexim1 Polyclonal Antibody detects endogenous levels of Hexim1 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitously expressed with higher expression in placenta, HEXIM1 and HEXIM2 are differentially expressed, Expressed in endocrine tissues,

Observed Band (KD): 40kD

Function: Domain: The coiled-coil domain mediates oligomerization
Function: Transcriptional regulator which functions as a general RNA polymerase II transcription inhibitor. In cooperation with 7SK snRNA sequesters P-TEFb in a large inactive 7SK snRNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation. May also regulate NF-kappa-B, ESR1, NR3C1 and CIITA-dependent transcriptional activity
Induction: Up-regulated by HMBA (hexamethylene bisacetamide) (at protein level). Down-regulated by estrogen
Miscellaneous: Inhibits Tat activity which is required for HIV-1 transcription
Similarity: Belongs to the HEXIM family
Subcellular Location: Binds alpha-importin and is mostly nuclear
Subunit: Homooligomer and heterooligomer with HEXIM2, probably dimeric. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, H

Subcellular Location: Nucleus Cytoplasm Binds alpha-importin and is mostly nuclear (PubMed:16362050)

Storage and Stability: -20°C/1 year

Gene Name: HEXIM1

Protein Name: Protein HEXIM1

Mouse Gene ID: 192231

Mouse SwissProt Number: Q8R409

Rat Gene ID: 498008

Rat SwissProt Number: Q5M9G1

Research Use Only