Product Description

HIF1 alpha Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BZ16362-50, BZ16362-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide, pH 7.3.

Swiss-Prot: Q16665

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: ICC/IF, WB, IHC-F, IHC-P, ELISA

All Applications: WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000

Background: Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1.

Purification and Purity: Affinity Purified

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: IgG

Bioworld Molecular Weight: Calculated MW: 93 kDa; Observed MW: 92-130 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of HIF1 alpha in LOVO lysates using HIF1 alpha antibody., Immunohistochemistry analysis of paraffin-embedded mouse colon tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval., Immunohistochemistry analysis of paraffin-embedded rat lung tissue using HIF1 alpha antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Alternative Name: HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; HIF-1-alpha; HIF1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; bHLHe78; Member of PAS protein 1; PAS doma

Immunogen: The antiserum was produced against synthesized peptide derived from human HIF-1alpha. AA range:328-377

Conjugate: Unconjugated

Modification: Unmodified