High Sensitive ELISA Kit for Melatonin (MT)
Sizes: 24T, 48T, 96T
Catalogue Numbers: HEA908Ge-24, HEA908Ge-48, HEA908Ge-96
Citations, Manuals and MSDS Available upon request.
Featured Series: High sensitivity kit
Target: Melatonin
Alternative Names: N-Acetyl-5-Methoxytryptamine
Target Species: General species
Featured Series Function: Detects protein (low-target protein, high-sensitivity)
Specificity: Reactive with General species MT / Melatonin
Method: Colormetric
Detection principle: Competitive Inhibition
Detection
range: 7.8-500pg/mL
Sensitivity: 2.8pg/mL
Assay Time: 3h
Sample Size: 50uL
Recommended/Predicted
Sample Types: Serum, Plasma and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Melatonin (MT).
No significant cross-reactivity or interference between High Sensitive Melatonin (MT) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Melatonin (MT) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Melatonin (MT) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to High Sensitive Melatonin (MT) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled High Sensitive Melatonin (MT) and unlabeled High Sensitive Melatonin (MT) (Standards or samples) with the pre-coated antibody specific to High Sensitive Melatonin (MT). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of High Sensitive Melatonin (MT) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of High Sensitive Melatonin (MT) in the sample.
Research Area: Metabolic pathway; Tumor immunity; Neuro science; Hormone metabolism;
Research Use Only