High Sensitive ELISA Kit for Nerve Growth Factor (NGF)
Sizes: 24T, 48T, 96T
Catalogue Numbers: HEA105Hu-24, HEA105Hu-48, HEA105Hu-96
Citations, Manuals and MSDS Available upon request.
Featured Series: High sensitivity kit
Target: Nerve Growth Factor
Alternative Names: NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor
Target Species: Human
Uniprot: P01138
Gene ID: 4803
Featured Series Function: Detects protein (low-target protein, high-sensitivity)
Specificity: Reactive with Human NGF / Nerve Growth Factor
Method: Colormetric
Detection principle: Double-antibody Sandwich
Detection
range: 3.12-200pg/mL
Sensitivity: 1.22pg/mL
Assay Time: 3h
Sample Size: 100uL
Recommended/Predicted
Sample Types: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of High Sensitive Nerve Growth Factor (NGF).
No significant cross-reactivity or interference between High Sensitive Nerve Growth Factor (NGF) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level High Sensitive Nerve Growth Factor (NGF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level High Sensitive Nerve Growth Factor (NGF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Target: 1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Sensitive Nerve Growth Factor (NGF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Sensitive Nerve Growth Factor (NGF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Sensitive Nerve Growth Factor (NGF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of High Sensitive Nerve Growth Factor (NGF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Cytokine; Neuro science;
Research Use Only