Product Description

High Sensitivity Human EGF (Epidermal Growth Factor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10240HS-48, ELK10240HS-96, ELK10240HS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: URG, Beta-Urogastrone, Pro-epidermal growth factor

Sensitivity: 1.44 pg/mL

Assay Type: Sandwich

Standard: 250 pg/mL

Detection Range: 3.91-250 pg/mL

Sample Type: Serum, Plasma

Assay Length: 3.5h

Research Area: Cytokine, Tumor Immunity, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EGF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P01133

Research Use Only