His-tag Polyclonal Antibody - BT-AP00862

His-tag Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP00862-20, BT-AP00862-50, BT-AP00862-100

Citations, Manuals and MSDS Available upon request.

Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Research Areas: Tags and cell markers; Signal transduction; Epigenetics and nuclear signaling

Target Protein: His-Tag

Applications: WB, ELISA

Reactivity: Species independent

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: His Tag

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Synonyms: His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Research Use Only