Product Description

His-tag Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1076-50, ES1076-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.

Reactivity: Species independent

Host: Rabbit

Applications: WB, ELISA

Alternate Names: 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; His tag

Clonality: Polyclonal

Immunogen: 6X His synthetic peptide conjugated to KLH.

Isotype: IgG

Target: His-tag,

Specificity: His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.

Dilution: Western Blot: 1/1000 - 1/3000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.

Observed Band (KD): 15kD

Storage and Stability: -20°C/1 year

Gene Name: His-Tag

Protein Name: His Tag

Research Use Only