Product Description

HisRS (E492) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS1927-50, BS1927-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P12081

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC, IF

All Applications: IHC: 1:50~1:200
IF: 1:50~1:200

Background: Histidyl-tRNA synthetase (HisRS, Jo-1) is an aminoacyl-tRNA synthetase that charges transfer RNAs (tRNAs) with a cognate histidine residue to produce histidyl-tRNA. Histidyl-tRNA is essential for the incorporation of histidine into proteins. Systemic autoimmune diseases lead to aminoacyl-tRNA synthetase autoantibodies that are directed against self structures. HisRS is an antigen in systemic autoimmune diseases such as rheumatic arthritis or polymyositis with associated interstitial lung disease, thrombocytopenia and Raynaud’s phenomenon (vasospastic attacks that cause blood vessels to constrict). HisRS autoantibodies are unlike anti-synthetase antibodies because they bind the nonlinear, exposed epitopes on native HisRS when the enzyme is complexed to tRNA(His).

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of HisRS (E492) pAb in paraffin-embedded human brain carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 60 kDa

Specificity: HisRS (E492) polyclonal antibody detects endogenous levels of HisRS protein

Alternative Name: Histidyl-tRNA synthetase; cytoplasmic; Histidine--tRNA ligase; HisRS; HARS; HRS

Immunogen: Synthetic peptide, corresponding to amino acids 361-410 of Human HisRS.

Conjugate: Unconjugated

Modification: Unmodified