Product Description

Histone H2A (Acetyl Lys15) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20537-50, ES20537-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: HIST1H2AG, H2AFP, HIST1H2AI, H2AFC, HIST1H2AK, H2AFD, HIST1H2AL, H2AFI, HIST1H2AM, H2AFN, Histone H2A type 1, H2A.1, Histone H2A/p, HIST2H2AA3, H2AFO, HIST2H2AA, HIST2H2AA4, Histone H2A type 2-A, Histone H2A.2, Histone H2A/o, HIST3H2A, Histone H2A type 3, H2AK15AC

Clonality: Polyclonal

Immunogen: Synthetic Peptide of Histone H2A (Acetyl Lys15)

Isotype: IgG

Target: Histone H2A

Specificity: The antibody detects endogenous Histone H2A (Acetyl Lys15) protein.

Dilution: WB: 1:500-1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Expression: Bone, Brain, Colon, Eye, Lymph, PCR rescued clones, Placenta, Sple

Observed Band (KD): 14kD

Fields: Necroptosis, Neutrophil extracellular trap formation, Alcoholism, Systemic lupus erythematosus

Function: Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling
mass spectrometry: Monoisotopic with N-acetylserine PubMed: 16457589,PTM: Deiminated on Arg-4 in granulocytes upon calcium entry
PTM: Monoubiquitination of Lys-120 by RING1 and RNF2/RING2 complex gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It is involved in the initiation of both imprinted and random X inactivation. Ubiquitinated H2A is enriched in inactive X chromosom

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: HIST1H2AG/HIST1H2AI/HIST1H2AK/HIST1H2AL/HIST1H2AM/HIST2H2AA3/HIST2H2AA4/HIST3H2A

Protein Name: Histone H2A type 1/Histone H2A type 2/Histone H2A type 3

Mouse Gene ID: 319164/15267/319162

Rat Gene ID: 365877/64646

Rat SwissProt Number: P02262/P0CC09/Q4FZT6

Research Use Only