Product Description

Histone H2A.X (phospho-S139) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS5023-50, BS5023-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: H2A.X is a member of the histone H2A family, 1 of 5 families of histone proteins involved in nucleosomal organization of chromatin. H2A.X is synthesised in G1 as well as S phase and is known to be important for recombination between immunoglobμlin switch regions. A very early step in the response of mammalian cells to DNA double-strand breaks is the phosphorylation of histone H2A.X at serine 139 at the sites of DNA damage. There is a predicted acetylation at residue 1 and ubiquitination at reside 119. Phosphorylated H2A.X promotes DNA repair and maintains genomic stability.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: Histone H2AX, H2a/x, Histone H2A.X, H2AFX, H2AX, γ-H2AX, γH2AX, ganunaH2AX, γ-H2A.X, γH2A.X, ganunaH2A.X

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human Histone H2A.X around the phosphorylation site of Serine 139.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-Histone H2A.X (S139) polyclonal antibody detects endogenous levels of Histone H2A.X protein only when phosphorylated at Ser139.

Molecular Weight: ~ 15 kDa

Pathway: Regμlation of Apoptosis, Histone Lysine Methylation Pathway,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P16104

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only