Product Description

Histone H2A.X (Phospho Thr120) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20579-50, ES20579-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-independent histone that is a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. [provided by RefSeq, Oct 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: H2AFX, H2AX, Histone H2A.x, H2a/x

Clonality: Polyclonal

Immunogen: Synthetic Peptide of Histone H2A.X (Phospho Thr120)

Isotype: IgG

Target: Histone H2A.X

Specificity: The antibody detects endogenous Histone H2A.X (Phospho Thr120) protein.

Dilution: WB: 1:1000-2000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Expression: Lung, Placenta,

Observed Band (KD): 15kD

Fields: Necroptosis, Neutrophil extracellular trap formation, Alcoholism, Systemic lupus erythematosus

Function: Developmental Stage: Synthesized in G1 as well as in S-phase
Domain: The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family
Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation
PTM: Mon

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: H2AFX

Protein Name: Histone H2A.x

Mouse Gene ID: 15270

Mouse SwissProt Number: P27661

Research Use Only