Product Description

Histone H2A.Z (Acetyl Lys7) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES20532-50, ES20532-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent member of the histone H2A family that is distinct from other members of the family. Studies in mice have shown that this particular histone is required for embryonic development and indicate that lack of functional histone H2A leads to embryonic lethality. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB

Alternate Names: H2AFZ, H2AZ, Histone H2A.Z, H2A/z

Clonality: Polyclonal

Immunogen: Synthetic Peptide of Histone H2A.Z (Acetyl Lys7)

Isotype: IgG

Target: Histone H2A.Z

Specificity: The antibody detects endogenous Histone H2A.Z (Acetyl Lys7) protein.

Dilution: WB: 1:500-1000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Formlation: PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

Expression: Brain, Epithelium, Skeletal muscle, Uterus,

Observed Band (KD): 14kD

Fields: Necroptosis, Neutrophil extracellular trap formation, Alcoholism, Systemic lupus erythematosus

Function: Function: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division
mass spectrometry: Monoisotopic, not modified PubMed: 16457589,PTM: Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis
PTM: Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression
PTM: Not phosphor

Subcellular Location: Nucleus Chromosome

Storage and Stability: -20°C/1 year

Gene Name: H2AFZ

Protein Name: Histone H2A.Z

Mouse Gene ID: 51788

Mouse SwissProt Number: P0C0S6

Rat Gene ID: 58940

Rat SwissProt Number: P0C0S7

Research Use Only