Product Description

Histone H3 (Acetyl-K18) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS70006-50, BS70006-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27 and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28 and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation of Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation of H3 Thr3 in prophase and its dephosphorylation during anaphase.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF

Alternate Names: Histone H3.1, HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD, HIST1H3F, H3FI, HIST1H3G, H3FH, HIST1H3H, H3FK, HIST1H3I, H3FF, HIST1H3J, H3FJ, Histone H3.2, Histone H3/m, Histone H3/o, HIST2H3A, HIST2H3C, H3F2, H3FM, HIST2H3D, Histone H3.3, H3F3A, H3.3A, H3F3, H3F3B, H3.3B

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Acetylated

Immunogen: Synthetic peptide including acetyl-lysine 18 of Histone H3.

Dilution: WB: 1:500~1:2000
IHC: 1:50~1:200
IF: 1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Ac-Histone H3 (K18) polyclonal antibody detects endogenous levels of Histone H3 protein only when acetylated at Lys18.

Molecular Weight: ~ 17 kDa

Pathway: Histone Lysine Methylation Pathway, Examples of Crosstalk Between Post-translational Modifications, DNA Methylation,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P68431/Q71DI3/P84243

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only