Product Description

Histone H3 (Acetyl Lys9) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1088-50, ES1088-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3;H3k9AC

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Histone H3 around the acetylated site of Lys9. AA range:3-52

Isotype: IgG

Target: Histone H3,

Specificity: Acetyl-Histone H3 (K9) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when acetylated at K9.

Dilution: WB 1:500-2000, IHC-p 1:50-300, IF 1:50-300

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Blood, Epithelium, Kidney, Lung, Ovary, Spleen, Uterus,

Observed Band (KD): 17kD

Fields: Neutrophil extracellular trap formation, Alcoholism, Shigellosis, Transcriptional misregulation in cancer, Systemic lupus erythematosus

Function: Caution: Was originally (PubMed:2587222) thought to originate from mouse. Developmental Stage: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. mass spectrometry:Monoisotopic with N-acetylserine PubMed:16457589, miscellaneous:This histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2). PTM: Acetylation is generally l

Subcellular Location: Nucleus. Chromosome.

Storage and Stability: -20°C/1 year

Gene Name: HIST1H3A/HIST1H3B/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B

Protein Name: Histone H3.1/Histone H3.2/Histone H3.3

Human Gene ID: 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/126961/333932/653604/3020/3021

Human SwissProt Number: P68431/Q71DI3/P84243

Mouse Gene ID: 319152/15077/15078

Rat Gene ID: 291159/100361558

Rat SwissProt Number: Q6LED0/P84245

Research Use Only